Day Neutral Flowering (DNF) of Autoflowering Cannabis

Son of Hobbes

High of the Tiger
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Oct 8, 2012
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I've been reading up on day neutral flowering (plants that have a flowering process that is not regulated by day length at all, but bloom when they are simply old enough to.)

From what I've found, there is a specific gene (or gene protein) that controls this, DAY NEUTRAL FLOWERING (DNF), which is a "membrane-bound E3 ligase involved in the regulation of flowering time."


I found this example about Arabidopsis thaliana (thale cress) :


The photoperiodic response in Arabidopsis thaliana requires the precise regulation of CONSTANS (CO) expression in relation to the light period during the day. In short days (SDs) levels of CO expression are normally low during the light period, and this results in delayed flowering compared with long days (LDs) when CO expression rises to high levels before the end of the light period. We identified a novel flowering time gene called DAY NEUTRAL FLOWERING (DNF) that acts in the same flowering pathway as CO. DNF is a membrane-bound E3 ligase that represses CO expression and plays an important role in maintaining low levels of CO expression in SDs. The effect of DNF on the rhythm of CO expression is essential for the photoperiodic response of Arabidopsis, enabling it to have a different flowering response in LDs and SDs.


"The photoperiod pathway channels inputs from light, day length, and the circadian clock to promote the floral transition. CONSTANS (CO) is a central regulator of this pathway, triggering the production of the mobile florigen hormone FT (FLOWERING LOCUS T) that induces flower differentiation. "

I found this article that talks about CONSTANS (CO) regulating flowering growth in cannabis: