Dec 9 update
Mephisto Fugue State pollen plant chopped yesterday, and mother plant had one branch pollenated twice today.
Mephisto 24C pollen plant chopped three days ago, and one branch of each pheno were pollenated a couple times thereafter.
All pollenation is now complete. Pistils on both pollenated branches of the 24C phenos are pretty much 100% curled and amber, so seed production should be good.
Lessons learned:
1. Injection with syringe works extremely well and quickly, apparently achieving virtually 100% pollenation of available pistils, and seems doable with little or no risk of stray pollen.
However:
2. Pumping syringe plunger has to be done under control to avoid pulling handle out of rubber seal and spreading pollen everywhere. This happened once with the 24C injections.
3. The syringe needle can go through both the top and then out through the side, leaving the tip exposed in the growdrobe. If one pumps the syringe when in this state, the entire bloody grow may end up pollenated. I may have done this with the Fugue State injection, so I will need to watch pistils to see what damage has been done. The same screwup is possible if during pumping the needle is accidentally pulled out of the isolation bag and then pumped. I might have done this once during 24C injection. Considering the screwups, I expect extra seeds.
4. The best pollenation bag is just close woven cloth, don't bother with plastic window, just remove the bag after several hours for pollen to "germinate" before misting the entire area down and removing the bag. Mist the inside of the bag thoroughly before pulling it off as well, pull it off gently and slowly while misting the area exposed as the bag is removed. Make sure all fans are off while this is being done, and while doing injections.
5. To reduce chance of pollen escape, mist all sides of the isolation bag before doing injection. This is a bit easier if done before bag is installed on the target branch.
Mephisto 24C pollen plant chopped three days ago, and one branch of each pheno were pollenated a couple times thereafter.
All pollenation is now complete. Pistils on both pollenated branches of the 24C phenos are pretty much 100% curled and amber, so seed production should be good.
Lessons learned:
1. Injection with syringe works extremely well and quickly, apparently achieving virtually 100% pollenation of available pistils, and seems doable with little or no risk of stray pollen.
However:
2. Pumping syringe plunger has to be done under control to avoid pulling handle out of rubber seal and spreading pollen everywhere. This happened once with the 24C injections.
3. The syringe needle can go through both the top and then out through the side, leaving the tip exposed in the growdrobe. If one pumps the syringe when in this state, the entire bloody grow may end up pollenated. I may have done this with the Fugue State injection, so I will need to watch pistils to see what damage has been done. The same screwup is possible if during pumping the needle is accidentally pulled out of the isolation bag and then pumped. I might have done this once during 24C injection. Considering the screwups, I expect extra seeds.
4. The best pollenation bag is just close woven cloth, don't bother with plastic window, just remove the bag after several hours for pollen to "germinate" before misting the entire area down and removing the bag. Mist the inside of the bag thoroughly before pulling it off as well, pull it off gently and slowly while misting the area exposed as the bag is removed. Make sure all fans are off while this is being done, and while doing injections.
5. To reduce chance of pollen escape, mist all sides of the isolation bag before doing injection. This is a bit easier if done before bag is installed on the target branch.
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