Seedling Veinal chlorosis, ongoing problem with multiple setups

I know it's kind of late, and maybe your meter is different but I thought that distilled water is bad news for any pH measuring device.
Thats not true, distilled water at 68° should read 7.0. My meter actually suggests storing in distilled water if you use it regularly and only storing in actually storing fluid if theres weeks between uses.
 
Thats not true, distilled water at 68° should read 7.0. My meter actually suggests storing in distilled water if you use it regularly and only storing in actually storing fluid if theres weeks between uses.
Ok, I took this directly from their (bluelabs) website:

"De-ionized, distilled, Pure or RO (reverse osmosis) water should never be used to store or hydrate a pH probe or pen. Pure water will affect the chemistry of your reference solution inside of your pH probe, and this will cause the probe or pen to fail prematurely."

Of course follow your manufacturers directions for your ph meter.
 
Ok, I took this directly from their (bluelabs) website:

"De-ionized, distilled, Pure or RO (reverse osmosis) water should never be used to store or hydrate a pH probe or pen. Pure water will affect the chemistry of your reference solution inside of your pH probe, and this will cause the probe or pen to fail prematurely."

Of course follow your manufacturers directions for your ph meter.
Screenshot_20231123_204802_Chrome.jpg

The detailed instructions say distilled water.


Edit, you are right, it does say to not use distilled water. Just regular water. I completely misread that.
 
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Problem: Veinal chlorosis on first true leaves, roughly 4 days from sprout

Medium/grow method: Autopots, but never get old enough to turn on, so: Hand watering. Multiple mediums tested. Cultivation Nation + additional perlite, Promix HPCC, Happy Frog, and as a last resort Miracle Gro potting mix because it normally makes anything grow for a while

Feed: and supplements used: PH ranging from 5.5 up to 7.0 for multiple tests. PPM from 30 (storebought distilled water only) up to 1000 just to see if I can get any sort of change. Greenleaf Megacrop 1 part, GenHydro FloraNova, GenHydro Flora series Grow, Nature's Living Soil Autoflower Concentrate. I have used all of these in different tests - not together. Nothing changes outcome.

water source: Tap - 300ppm 7.4ph, store bought distilled - 30ppm 7.0ph, water from friend's well - 150ppm, 7.2ph

Strain/age: Many different Mephisto Genetics strains, too many to remember. Also tried Ethos Pluto Runtz Auto and Purple Thai Auto

light used: Mars Hydro FCE6500, Enjoyield diy240, both tried on separate tests with differing amounts of power and distance from plants, use photone app to check dli, dli ranging from 5-20 on different tests, no changes other than at low dli seedlings stretch

Climate: Using ac infinity controller 69 pro, have tried VPD ranging from .8-1.2

Additional info: This has been ongoing for 4 months now, every seed I germinate via the paper towel method, when a tap root comes out I put in the pots in the tent. After 4 days or so veins begin yellowing, and continue to progress. Plant is very slow to grow if at all. Does not respond to nutrients, I can feed them an obviously overdose amount of nutrients with absolutely no change. I have repeatedly each week popped new seeds to test out new things and every week it is the same, roughly day 4 above ground symptoms start. Used 4 different soils, amended, non amended, peat to completely inert coco mixes. Have used 3 different water sources, dechlorinated tap, distilled, well water from friend's house. All of these water sources have been tested non adjusted and adjusted to different ph's over the months of this problem, 5.5 ph going in does no different than not ph'ing my tap water and putting it in at 7.4. Water amounts have been tested at multiple levels. Have tried soaking entire medium with water on planting, all the way down to intentionally underwatering.

A little background, this is supposed to be my 3rd grow in this tent if I can ever get something to grow. It's been 4 months of trying every variable I can think of. Every seed I plant ends up looking like the pictures. In one of the pictures I have a plant in the background showing advanced stages of the problem. Posting more pictures would only be redundant, no matter what I try they all do the same thing. I've tried 4 different soil mixes, tried amending some with NLS autoflower concentrate. Tried 3 different types of water, running all the way from completely base non ph adjusted water, to ph'd down to 5.5 at the lowest, no nutrients all the way up to 1000ppms trying to get anything to change, no changes observed. Tried watering as little as a tablespoon on initial plant and waiting until the plant shows drooping from lack of water, all the way up to soaking medium. Megacrop 1 part and genhydro flora grow and floranova bloom used separately in different tests ranging from 100ppm up to 1000ppm.

The autopots are the new addition to the tent this grow, but I am not even getting to the point where I can turn them on, they're no different than a big plastic pot. I've also tried solo cups and ac infinity cloth pots to rule out something in the plastic from autopots, no change.

I have tried so many different things, because at this point I want to see anything different. Nothing changes, I could show a hundred pictures of different seedlings over these 4 months and they all look exactly the same no matter differing variable of that seedling. I have wasted around 50 seeds so far. It's disheartening I've never had problems at least getting any type of plant to grow, I've been gardening with my mom since I could walk, so nearly 40 years now. What am I missing? Why is this run failing so early? Any help would be greatly appreciated.
I'm new though recently made a mistake adding too much P to my seedlings thinking they would root better. Now they are Iron deficient. I viewed some UConn ag video on this and excess P will block ability to absorb N. Here's and example from UConn

Is your soil brand new or refreshed? This looks a lot like my pepper seedlings from a few weeks ago. And a whole container crop of radishes just went from great to pale leaves w little to no growth. They are blocked from N and I have some Chelated Iron product to help get them greened up.
Good luck !
Anyway, something to consider.
 

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