Cloning autos, the key may be tissue culture!

[pop22]

This needs more looking into, but I've always suspected that tissue culture was the key t cloning autos. There is an excellent article on growweedeasy on tissue culture and her mentions that it works for autos and seems to reset the clock! No direct link to auto information was included but I'm looking into this more. I had always thought that using a very young seedling might be the key but it seems that using bud fragments holds the answers.

https://www.growweedeasy.com/live-plant-cannabis-tissue-culture

 

[Son of Hobbes]

This needs more looking into, but I've always suspected that tissue culture was the key t cloning autos. There is an excellent article on growweedeasy on tissue culture and her mentions that it works for autos and seems to reset the clock! No direct link to auto information was included but I'm looking into this more. I had always thought that using a very young seedling might be the key but it seems that using bud fragments holds the answers.

https://www.growweedeasy.com/live-plant-cannabis-tissue-culture

There are some commercial grows using tissue culturing here in Colorado, but it's not common (I suspect it's expensive, and the requirements for sterility ixnays a lot of grows immediately.)

@derek420colorado and I have talked quite a bit about it for autoflowers; I'm sure we could find some companies out there that offer kits and see if we can't get some testing done?

 

[Bob's Auto's]

This needs more looking into, but I've always suspected that tissue culture was the key t cloning autos. There is an excellent article on growweedeasy on tissue culture and her mentions that it works for autos and seems to reset the clock! No direct link to auto information was included but I'm looking into this more. I had always thought that using a very young seedling might be the key but it seems that using bud fragments holds the answers.

https://www.growweedeasy.com/live-plant-cannabis-tissue-culture

Interesting read as usual Pop

 

[Son of Hobbes]

I have multiple tissue culturing companies pulled up on my computer; I'll try to reach out to them this week. It doesn't look super complex.

 

[WildBill]

I've been seeing postings on IG on this subject.

 

[Bill.de.Cat]

Wondering if the whole process is needed to "reset" an AF or if just the rooting part would reset it.

 

[kfigerm]

you are so awesome guys..afn always upfront and digging into things!
I'm so happy that i found my way back to you faboulous guys and gals!

 

[pop22]

I suspect it's the process of making the exoplant that does the reset.

Wondering if the whole process is needed to "reset" an AF or if just the rooting part would reset it.

 

[pop22]

Maybe you can get oone of them to sponsor some tests here on AFN!

I have multiple tissue culturing companies pulled up on my computer; I'll try to reach out to them this week. It doesn't look super complex.

 

[Son of Hobbes]

Maybe you can get oone of them to sponsor some tests here on AFN!

That's the plan!

Hemp Multiplication Kit

The purpose of this kit is to demonstrate in vitro vegetative propagation. This kit provides the necessary materials to initiate cultures from the seeds and/or stem tissues of hemp. Plant tissues are not included in kit and must be supplied by the user.

phytotechlab.com

Microclone Propagation & Tissue Culture

Helping small to large gardeners' tissue clone clean, disease free, vigorous plants.

microclone.com

Plant Cell Technology

Plant Cell Technology manufactures PPM™ (Plant Preservative Mixture), a heat stable preservative/biocide that effectively prevents or reduces microbial and fungal contamination in plant tissue culture

www.plantcelltechnology.com